Developmental competence of Dromedary camel oocytes fertilized in vitro by frozen-thawed ejaculated and epididymal spermatozoa

نویسندگان

  • D. A. El-Badry Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt
  • K. Gh. M. Mahmoud Department of Animal Reproduction and Artificial Insemination, National Research Center, Dokki, Giza, Egypt
  • T. H. Scholkamy Department of Field Investigations, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt
چکیده مقاله:

The present study aimed to compare the in vitro fertilizing capacity of frozen-thawed ejaculated and epididymal spermatozoa in order to standardize the semen preparation protocol for camel in vitro fertilization (IVF). Semen samples were collected from 7 Dromedary camels by means of artificial vagina (AV). Ten cauda epididymes were obtained from slaughtered adult camels, isolated, incised and rinsed for obtaining the sperm rich fluid. Ejaculated and epididymal spermatozoa were processed for cryopreservation. Fresh and frozen-thawed ejaculated and epididymal spermatozoa were evaluated for motility, livability, membrane and acrosomal integrities. Frozen-thawed ejaculated and epididymal spermatozoa were used to fertilize camel mature oocytes in vitro. The results showed that, the progressive motility of freshly collected epididymal spermatozoa was significantly (P

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عنوان ژورنال

دوره 17  شماره 4

صفحات  253- 258

تاریخ انتشار 2016-12-24

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